Lysate in a sentence
Synonym: extract.
Meaning: A solution containing the contents of lysed cells.
(1) The lysate was thawed on ice before use.
(2) The lysate was loaded onto a gel for electrophoresis.
(3) The lysate was cooled on ice before further analysis.
(4) The lysate contained a high concentration of proteins.
(5) The lysate was then subjected to Western blot analysis.
(6) The lysate was sonicated to disrupt the cell membranes.
(7) The lysate was loaded onto a gel for SDS-PAGE analysis.
(8) The lysate was aliquoted into smaller volumes for storage.
(9) The lysate was prepared by lysing the cells with a detergent.
(10) The lysate was visualized using a chemiluminescent substrate.
Lysate sentence
(11) The lysate was treated with RNase to degrade any RNA present.
(12) The lysate was concentrated using a centrifugal filter device.
(13) The lysate was sonicated on ice to disrupt the cell membranes.
(14) The lysate was treated with a nuclease to remove nucleic acids.
(15) The lysate was transferred onto a membrane for protein blotting.
(16) The lysate was mixed with a loading dye for gel electrophoresis.
(17) The lysate was prepared by disrupting the cells using a sonicator.
(18) Deproteinize the cell lysate before measuring the enzyme activity.
(19) The researchers quantitated the amount of protein in the cell lysate.
(20) The lysate was incubated with a chelating resin to remove metal ions.
Lysate make sentence
(21) The lysate was treated with a reducing agent to break disulfide bonds.
(22) The lysate was incubated with an antibody to detect the target protein.
(23) The lysate was treated with a nuclease to remove any DNA contamination.
(24) The experiment requires us to remove glycoprotein from the cell lysate.
(25) The lysate was analyzed by mass spectrometry for protein identification.
(26) The researcher used an enzymatic method to deproteinize the cell lysate.
(27) The lysate was clarified by centrifugation at 14,000 rpm for 15 minutes.
(28) The lysate was treated with a detergent to solubilize membrane proteins.
(29) The lysate was sonicated in a lysis buffer to release cellular contents.
(30) The lysate was diluted with a buffer to adjust the protein concentration.
Sentence of lysate
(31) The lysate was analyzed by Western blotting to detect the target protein.
(32) The lysate was centrifuged at high speed to pellet the insoluble material.
(33) The lysate was incubated with a fluorescent dye to visualize protein bands.
(34) The lysate was treated with a DNAse enzyme to remove any DNA contamination.
(35) The lysate was then incubated with an antibody to capture the target protein.
(36) The protein concentration in the lysate was determined using a Bradford assay.
(37) The lysate was centrifuged at 10,000 rpm for 10 minutes to remove cell debris.
(38) The lysate was subjected to immunoprecipitation to isolate the target protein.
(39) The lysate was digested with a specific protease to generate peptide fragments.
(40) The lysate was aliquoted into microcentrifuge tubes for individual experiments.
Lysate meaningful sentence
(41) The lysate was treated with a protease inhibitor to prevent protein degradation.
(42) The lysate was probed with a secondary antibody conjugated to a fluorescent dye.
(43) The lysate was supplemented with a reducing agent to maintain protein stability.
(44) The lysate was incubated with a metal chelating resin for affinity purification.
(45) The first step in the experiment is to remove glycoprotein from the cell lysate.
(46) The researchers were able to isolate an oligomeric complex from the cell lysate.
(47) The bioreagent was used to measure the activity of the enzyme in the cell lysate.
(48) The researchers used centrifugation to isolate glycoprotein from the cell lysate.
(49) The lysate was analyzed using a Bradford assay to determine protein concentration.
(50) The cell lysate must incubate prior to being centrifuged for protein purification.
Lysate sentence examples
(51) The lysate was analyzed using a spectrophotometer to measure protein concentration.
(52) The researcher used trichloroacetic acid to precipitate proteins from a cell lysate.
(53) Lysing is often followed by centrifugation to separate cellular debris from the lysate.
(54) The lysate was diluted with a lysis buffer to achieve the desired protein concentration.
(55) The lysate was treated with a phosphatase inhibitor to preserve phosphorylated proteins.
(56) The lysate was mixed with a loading buffer and boiled for 5 minutes prior to gel loading.
(57) The team employed a Western blotting technique to detect glycoproteins in the cell lysate.
(58) The scientist employed a differential centrifugation technique to fractionate the cell lysate.
Lysate meaning
Lysate is a term that is commonly used in the field of biology and biochemistry. It refers to a solution that is obtained by breaking open cells and releasing their contents. This solution contains a mixture of proteins, nucleic acids, and other cellular components that can be used for various research purposes. If you are working in the field of biology or biochemistry, it is important to know how to use the word lysate correctly in a sentence. Here are some tips to help you use this term effectively:
1. Understand the meaning of lysate Before you can use the word lysate in a sentence, it is important to understand what it means. As mentioned earlier, lysate refers to a solution that is obtained by breaking open cells and releasing their contents. This solution can be used for various research purposes, such as protein purification, enzyme assays, and DNA extraction.
2. Use lysate in the appropriate context When using the word lysate in a sentence, it is important to use it in the appropriate context.
For example, if you are discussing a protein purification experiment, you might say, "After centrifugation, the lysate was collected and loaded onto a column for purification." This sentence clearly indicates that the lysate was obtained from cells and was used for protein purification.
3. Use lysate as a noun Lysate is a noun, which means it should be used as the subject or object of a sentence.
For example, you might say, "The lysate was analyzed using SDS-PAGE to determine the purity of the protein." In this sentence, lysate is the subject of the sentence, and the verb is analyzed.
4. Use lysate with appropriate adjectives When using lysate in a sentence, it is important to use appropriate adjectives to describe it.
For example, you might say, "The bacterial lysate was highly concentrated and contained a large amount of protein." In this sentence, the adjectives bacterial, highly concentrated, and large amount of protein provide additional information about the lysate.
5. Use lysate in the plural form When referring to multiple lysates, it is important to use the plural form of the word.
For example, you might say, "The lysates were analyzed using Western blotting to detect the presence of specific proteins." In this sentence, lysates is the subject of the sentence, and the verb is analyzed.
In conclusion, lysate is a term that is commonly used in the field of biology and biochemistry. To use this term effectively in a sentence, it is important to understand its meaning, use it in the appropriate context, use it as a noun, use appropriate adjectives, and use it in the plural form when referring to multiple lysates. By following these tips, you can effectively communicate your research findings and ideas to others in the field.
The word usage examples above have been gathered from various sources to reflect current and historical usage of the word Lysate. They do not represent the opinions of TranslateEN.com.